PROBLEM TO BE SOLVED: To obtain a novel H gene of canine distemper virus which possesses the base sequence encoding H protein containing a specific amino acid sequence or the base sequence giving a specific restriction enzyme digestive fragment and is effective in the prophylaxis, treatment, examination or the like of canine distemper virus infectious diseases.SOLUTION: This novel H gene of canine distemper virus possesses the base sequence encoding the amino acid sequence of H protein represented by the formula or the base sequence which encodes a polypeptide showing the amino acid sequence homology of more than 95% to this amino acid sequence and gives two kinds of fragments when digested with restriction enzyme NdeI or PshBI and is effective in the prophylaxis, treatment, examination or the like of distemper virus infectious diseases on a mammal, e.g. dog or the like. This H gene is obtained by isolating whole RNA from distemper virus by a widely known method, synthesizing cDNA by using the RNA as a template and cloning the cDNA by PCR method using a primer consisting of the partial sequence.COPYRIGHT: (C)2000,JPO【課題】 新規なイヌジステンパーウイルスのH遺伝子を提供でき、イヌ等の哺乳動物におけるジステンパーウイルス感染症の予防/治療/検査に有効な新規なH遺伝子、それがコードするHタンパク質及びそれを含む新規なイヌジステンパーウイルスを提供すること。【解決手段】 特定のHタンパク質のアミノ酸配列をコードする塩基配列、あるいは該アミノ酸配列と95%以上のアミノ酸配列相同性があるポリペプチドをコードし、制限酵素NdeI又はPshBIで消化すると2種の断片が得られる塩基配列を有するイヌジステンパーウイルスのH遺伝子、それを含むイヌジステンパーウイルス、及びHタンパク質。
