An adjustable tree stand assembly of the present invention is used by hunters and naturalists. The assembly presents a support frame of a generally rectangular configuration having a first support member to be connected to a tree and an arm extending in cantilevered fashion to a housing adaptable to receive a seat and seat frame assembly. The seat and seat frame assembly includes a seat portion, a back support portion and a leg or pin extending from the seat portion to be engaged in the housing. An adjustable shooting rail is connected to and extends from the seat and seat frame assembly. The housing is further connected to a platform for supporting the hunter.