The method is intended for use in the healthcare, in particular for selective disinfection against agents causative of healthcare-associated infections (HCAIs).To provide the selective disinfection, an agent-specific bacteriophage with a lytic activity of 10-4 to 10-9 and phage content of at least 105 per 1 ml of the product is applied in the amount of 1 to 2 ml/m2 onto the object to be treated.The biological selective disinfection method utilizing bacteriophages provides a pronounced effect against Pseudomonasaeruginosa, Salmonellatyphimurium and other most common HCAI agents, does not cause any side effects, is environmentally safe and economically advantageous.