The invention provides an integrated sampling and detecting device with a misassembly prevention structure. The integrated sampling and detecting device with a misassembly prevention structure comprises: a detector a test strip holder, detachably arranged in the detector a collector, detachably contained in the detector a faeces collection piece and a cushion block, configured to detachably seal the collector and a piercing part, arranged on a bottom surface of the detector and configured to pierce the collector, wherein the test strip holder and the collector are contained in the detector side by side, the collector is located between the test strip holder and the detector, a first outer side face of the collector is adjacent to an inner side wall of the detector, a second outer side face of the collector is adjacent to the test strip holder, and the first outer side face and the second outer side face of the collector are asymmetric structurally. The invention solves the problem that a side, bonded with a bar code, of a collector is misassembled into a side, close to a test strip holder, of a detector, and also avoids a phenomenon of mis-bonding of a bar code.
