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Methods of obtaining human umbilical artery tissue cells after delivery
专利权人:
INC.;JANSEN BIOTECH
发明人:
申请号:
ARP180101473
公开号:
AR112356A1
申请日:
2018.06.01
申请国别(地区):
AR
年份:
2019
代理人:
摘要:
The request provides methods for obtaining cells from mammalian (preferably human) postpartum umbilical cord tissue, as well as the use of these cells for the treatment of ocular degeneration. 1. Claim 1: a method for producing human umbilical artery tissue cells from human umbilical cord tissue, including: (a) obtaining umbilical cord tissue; (b) removing all blood from tissue in large quantity to produce umbilical cord tissue without blood basically; (c) to separate human umbilical artery tissue from umbilical cord tissue;(d) the separation of umbilical artery tissues without blood by mechanical decomposition (such as grinding); (E) the digestion of separated tissues by mixed enzymes (including metalloprotein, neutron protein and mucilase); (f) the separation of cells from umbilical artery tissues; By digesting tissue; and (g) culturing cells to ensure the homogeneity of cells produced from isolated umbilical artery tissue, in which cells can self renew and grow, have the potential to differentiate themselves from other phenotype cells, andThe following characteristics: CD13, CD90, NG2 and HLA-ABC; CD31, CD34, CD45, CD117; FSP1 or e-caderina.La solicitud proporciona métodos para obtener células de tejido del cordón umbilical posparto de mamíferos, preferentemente, seres humanos y uso de estas células para tratar afecciones degenerativas oculares. Reivindicación 1: Un método para generar células derivadas de tejido arterial del cordón umbilical humano de tejido del cordón umbilical humano que comprende: (a) obtener tejido del cordón umbilical; (b) eliminar sustancialmente toda la sangre del tejido para producir tejido umbilical sustancialmente libre de sangre; (c) separar tejido arterial del cordón umbilical humano a partir de tejido del cordón umbilical; (d) disociar el tejido arterial del cordón umbilical humano sustancialmente libre de sangre mediante disociación mecánica tal como molienda; (e) digerir el tejido disociado con una mezcla de enzimas que comprenden
来源网站:
中国工程科技知识中心
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