GUPTA MANJU,NOVAK STEPHEN,WEBB STEVEN R.,HENRY MATTHEW J.,COGAN NOEL,HAYDEN MATTHEW,SAWBRIDGE TIM,SPANGENBERG GERMAN,AINLEY W. MIKE,MANSON JOHN,KUMAR SANDEEP,FORSTER JOHN
申请号：
CL2015000565
公开号：
CL2015000565A1
申请日:
2015.03.06
申请国别(地区):
CL
年份:
2015
代理人:
摘要：
An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5&prime； and 3&prime； ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.Se describe una Plataforma de Integración de Transgenes Modificados por Ingeniería Genética (ETIP) que puede insertarse en forma aleatoria o en lugares determinados en genomas de plantas para facilitar la rápida selección y detección de un gen de interés que es reconocido (tanto los extremos 5' como 3') en la ubicación genómica.
