VAN LEEUWEN, JOHANNES GUSTAAF ERNST,ZWARTJENS, PRISCILLA,BOER, VIKTOR MARIUS
申请号：
CA3045722
公开号：
CA3045722A1
申请日:
2017.12.04
申请国别(地区):
CA
年份:
2018
代理人:
摘要：
The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
