OBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOSTJU "BIOTEKHAGRO" (OOO "BIOTEKHAGRO")
发明人:
NOVIKOVA ELENA NIKOLAEVNA,Новикова Елена Николаевна,KOBA IGOR SERGEEVICH,Коба Игорь Сергеевич,KOPYLTSOV SERGEJ VASILEVICH,Копыльцов Сергей Васильевич,ZIMIN KONSTANTIN VIKTOROVICH,Зимин Константин Викт
申请号：
RU2012146497/15
公开号：
RU0002497534C1
申请日:
2012.10.31
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention relates to veterinary science. A method for preparing a bacterial preparation for preventing bovine postpartum endometritis consisting in the fact that preparing involves making a dry nutrient broth for microorganism culture (DNB) according to the package insert the prepared broth is added with 3% starch pre-dissolved in a small amount of the cooled broth, and then mixed with the rest DNB the broth is brought to the boiling point, boiled to produce a homogenous transparent mass then the medium is added with 0.125% NaCl and 2% glucose to pH 6-7, then a phosphate buffer is added: one-substituted 0.8% potassium KH2PO4 and 0.18% two-substituted potassium or sodium K2HPO4 or Na2HPO4 the solution is boiled for 15 minutes, and after cooling, it is added with 0.5% of 80% lactic acid, filtered and autoclaved at 0.5 atm. for 30-40 min after the solution has been cooled, it is added with the prepared cultures of Lactobacillus fermentum 44/1 and Lactococcus lactis subsp.Lactis 574 no 1 bln. of the microbial cells per 100 ml of the medium in the sterile environment it is packaged and thermostated for 48 hours at t=37°C. The preparation is tested in 56 cows for the purpose of preventing bovine postpartum endometritis.EFFECT: invention provides reducing the cost price of the preparation as compared to its prototype by 60%, without impairing the prophylactic efficacy, eliminates using antibiotics and derivatives thereof, sulphanyl amides, nitrofuranes and thereby eliminates side action of the preparation on animals body.1 tbl, 2 exИзобретение относится к области ветеринарии. Способ получения бактериального препарата для профилактики послеродового эндометрита у крупного рогатого скота заключается в том, что для приготовления препарата готовят питательный бульон для культивирования микроорганизмов сухой (СПБ) согласно инструкции по применению, к полученному бульону добавляют 3% крахмала, предварительно растворенного в небольшом количестве охлажден
