The present invention provides an Angelica dahurica ferment, preparation method thereof and use thereof for improving skin appearance. The Angelica dahurica ferment can effectively improve the water retention of the skin, enhance the brightness of the skin, and/or lighten the pigmentation of the skin to improve the dryness, lack of water and dullness of the skin, and make the skin look moist, clean, transparent, and shiny. The Angelica dahurica burning ferment is prepared by fermenting the Angelica dahurica extract to yeast, lactic acid bacteria, and acetic acid bacteria for three-stage fermentation.本發明提供一種白芷發酵物及其製備方法與用於改善皮膚外觀的用途,本發明之白芷發酵物能有效提升皮膚之保水度、提升皮膚之亮澤度、及/或淡化皮膚之色斑,以改善皮膚乾燥、缺水、暗沉的情況,使皮膚外觀水潤、淨透、且有光澤;其中,該白芷發酵物係將由一白芷萃取物所組成之混合物以酵母菌、胚芽乳桿菌、及醋酸桿菌依序進行三段式發酵而獲得。