This invention provides a method of producing Pseudomonas fluorescens using submerged fermentation under control condition of optimized batch parameters. The present invention relates to growing Pseudomonas fluorescens in liquid phase and it is method of scale up and mass multiplication. In present invention, the bacterial strain was also optimized for various factors like carbon nitrogen sources, temperature, pH, agitation and aeration etc. The invention also summarizes the most effective application methodology for suppressing plant diseases under natural field conditions.
