MACDONALD LYNN,MURPHY ANDREW J.,MCWHIRTER JOHN,MARTIN JOEL H.
申请号：
NZ62907713
公开号：
NZ629077A
申请日:
2013.03.15
申请国别(地区):
NZ
年份:
2015
代理人:
摘要：
Disclosed is a genetically modified non-human animal comprising in its germline an immunoglobulin locus that comprises an unrearranged immunoglobulin variable gene sequence comprising a substitution of at least one non-histidine codon with a histidine codon or an insertion of at least one histidine codon, wherein the substituted or inserted histidine codon is not encoded by a corresponding wild-type gene segment, and wherein the nonhuman animal expresses a nucleotide sequence encoding an immunoglobulin variable domain that comprises a histidine amino acid derived from the substitution of the at least one non-histidine codon or insertion of the at least one histidine codon in the unrearranged immunoglobulin variable gene sequence. Also disclosed are methods for making such a non-human animal.
