An aspiration apparatus is described that includes a guide and a set of needles. The guide preferably has an approximately conical shape with a decreasing diameter in the distal direction. A plurality of lumens extend between a distal end portion and a proximal end portion of the guide. The needles have a tubular wall and are slidingly positionable in the lumens. The needles include a longitudinal section of the tubular wall that has a plurality of apertures that decrease in size in the proximal direction. The apertures define an approximately conically shaped area of harvest. The aspiration apparatus can connect to an external source of reduced pressure through the guide or directly to each needle.