Disclosed are: a method for inducing the differentiation of stem cells into tissue cells a bioartificial organ which comprises the tissue cells and a material for medical purposes, which comprises the bioartificial organ. Stem cells capable of proliferation, self-replication and differentiation are used and cultured by a hanging-drop method to three-dimensionally construct a cell mass of embryoid bodies, and the cell mass is cultured in the presence of HGF, GDNF, b-FGF, BMP7 and EGF. In this manner, the stem cells can be differentiated into tissue cells. A bioartificial organ can be produced using cells that have been differentiated into the tissue cells. Further, a material for medical purposes can be provided.本發明在於提供一種從幹細胞分化誘導成組織細胞之方法,以及包含所得之組織細胞的生物人工內臟器官。進一步在於提供一種包含所製造之生物人工內臟器官的醫療用材料。解決方式為利用具有增殖能力、自我複製能力、分化能力的幹細胞並透過懸滴法(hanging drop method)來進行培養,藉此以三維方式建構胚體(embryonic body)細胞塊,並藉由在HGF、GDNF、b-FGF、BMP7及EGF的存在下,對所述細胞塊進行培養即可分化成組織細胞。又可使用分化成組織細胞的細胞來製造生物人工內臟器官,而能夠進一步提供醫療用材料。
