Disclosed are compositions and Methods for controlling plant pests.In particular, it provides Proteins having insecticidal toxicity against insects or Coleoptera and Lepidoptera.Also provided are Nucleic Acid molecules encoding insecticidal Proteins.Also disclosed are methods of preparing insecticidal proteins and Methods of use of insecticidal proteins and Nucleic Acids encoding insecticidal Proteins, for example,In transgenic plants to confer protection against Insect damage.Claim 1: an expression cassette comprising a functional Promoter linked to a heterologous Nucleic Acid Molecule.Comprising a Nucleotide sequence that includes: (a) a Nucleotide sequence of any of the SEQ ID no. 1 to 3 or SEQ ID no. 6 to 8； (b) a Nucleotide sequence that is at least80% identical to the Nucleotide sequence of any of the SEQ ID no. 1 to 3 or SEQ ID no. 6 to 8； (c) a Nucleotide sequence that encodes a polypeptide.Where the amino acid sequence of the polypeptide comprises SEQ ID no. 4, SEQ ID no. 5 or SEQ ID no. 9； (d) a Nucleotide sequence that encodes a polypeptide.Where the amino acid sequence of the polypeptide is at least 80% similar in the amino acid sequence of the SEQ ID no. 4SEQ ID no. 5 or SEQ ID no. 9； (e) a Nucleotide sequence that is complementary to the Nucleotide sequence of one of (a) to (d) above.Claim 24: a transgenic Plant which comprises a Nucleic Acid Molecule that confers Enhanced resistance to insectsWhere such a Nucleic Acid Molecule includes the expression cassette of claim 1.Se divulgan composiciones y métodos para el control de plagas de plantas. En particular, se proporcionan proteínas insecticidas que tienen toxicidad contra plagas de insectos coleópteros y/o lepidópteros. También se proporcionan moléculas de ácido nucleico que codifican las proteínas insecticidas. También se divulgan métodos de preparación de las proteínas insecticidas y métodos de uso de las proteínas insecticidas y ácidos nucleicos que codifican las proteínas insecticidas, por ejempl
