AINLEY, W. Michael,SPANGENBERG, German,WEBB, Steven R.,GUSCHIN, Dmitry Y.,HAYDEN, Matthew,ISENEGGER, Daniel,MASON, John,MILLER, Jeffrey C.,PETOLINO, Joseph F.,RAN, Yidong,SAWBRIDGE, Tim
申请号:
USUS2014/032706
公开号:
WO2014/165612A3
申请日:
2014.04.02
申请国别(地区):
US
年份:
2015
代理人:
摘要:
Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes. The present disclosure provides methods and compositions for precision transformation, gene targeting, targeted genomic modification and protein expression in plants. In particular, the present disclosure describes a novel, transgenic marker-free strategy for integrating an exogenous sequence and to stack traits that exploit differential selection at an endogenous locus, e.g., the acetohydroxyacid synthase (AHAS) locus) in plant genomes.La présente invention concerne des procédés et des compositions permettant un empilage parallèle ou séquentiel de transgènes chez des plantes afin d'obtenir des plantes présentant des phénotypes sélectionnés.