RANCH JEROME P,LIEBERGESELL MATTHIAS,GARNAAT CARL W,HUFFMAN GARY A
申请号：
IN3999/DELNP/2011
公开号：
IN2011DN03999A
申请日:
2011.05.27
申请国别(地区):
IN
年份:
2012
代理人:
摘要：
Auxotrophic Agrobacterium and methods employing auxotrophic Agrobacterium are provided. Auxotrophic Agrobacterium may be used in a variety of methods including biologically containing Agrobacterium comprising a transgene and transforming a plant cell without using an Agrobacterium counter-selective agent. Transforming maize immature embryos using an Agrobacterium auxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic Agrobacterium. Methods for producing an Agrobacterium thymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.
