Provided are a siRNA for suppressing hepatitis B virus gene expression, and a pharmaceutical composition and conjugate containing the siRNA. Each nucleotide in the siRNA is an independent modified nucleotide. The siRNA contains a positive-sense strand and an antisense strand. The positive-sense strand of the siRNA contains a nucleotide sequence 1 having an equal length to a nucleotide sequence shown in SEQ ID NO: 155 with no more than three nucleotide differences, and the antisense strand of the siRNA contains a nucleotide sequence 2 having an equal length to a nucleotide sequence shown in SEQ ID NO: 156 with no more than three nucleotide differences.
