Disclosed is a canine influenza recombinant virus. The recombinant virus comprises HA and NA genes of ZJCIV canine influenza virus as well as six internal genes PA, PB1, PB2, M, NP and NS of a PR8 virus. Also disclosed is a preparation method for the canine influenza recombinant virus and an application of the canine influenza recombinant virus. The canine influenza recombinant virus in the present invention can generate very high virus titer and hemagglutination titer on both a chicken embryo and an MDCK cell, and can be used as a good seed virus for developing canine influenza vaccines.
