The present invention provides a method for purifying an EV71 virus-like particle and a method for preparing a vaccine thereof. The virus-like particle is obtained by performing high density fermentation cultivation on recombinantly engineered bacteria； inducing expression of the EV71 virus-like particle protein expression using methanol； collecting the bacteria by centrifugation and performing high-pressure homogenization for disruption； performing precipitation on the supernatant with ammonium sulfate； and purifying by redissolution, ultrafiltration, ion exchange chromatography, molecular sieve chromatography, hydroxyapatite chromatography, etc.
