Problem to be solved: to provide a method of manufacturing a lung derived from a pluripotent stem cell produced in vivo of a non human mammalian.A method of manufacturing the lung is a step 1 for preparing pluripotent stem cells, a process for transplanting said pluripotent stem cells into a fertilized ovary of eight non - or blastocyst stages of non human mammals, and a process for generating the fertilized eggs in the fetal of a non human parental mammal and obtaining the birth and delivery of the fertilized egg from the product Steps 4 and 4 for obtaining a lung are provided in order, and the non human mammal is a non human mammal having an abnormality in which the generation of the lung does not occur in the developmental stage, and the pluripotent stem cell andThe lungs are from heterozygous mammals of individuals differing from the non human mammals, and are homologous or heterologous to the non human mammals and the heterozygous mammals.No selection【課題】非ヒト哺乳動物の生体内で作製された多能性幹細胞由来の肺を製造する方法を提供する。【解決手段】肺の製造方法は、多能性幹細胞を調製する工程1と、非ヒト哺乳動物の8細胞期又は胚盤胞期の受精卵中に前記多能性幹細胞を移植する工程2と、前記受精卵を非ヒト仮親哺乳動物の母胎中で発生させて、産仔を得る工程3と、前記産仔個体から肺を取得する工程4と、を順に備える方法であり、前記非ヒト哺乳動物は、発生段階において、肺の発生が生じない異常を有する非ヒト哺乳動物であり、前記多能性幹細胞及び製造される肺は、前記非ヒト哺乳動物と異なる個体の異個体哺乳動物由来であり、且つ、前記非ヒト哺乳動物と前記異個体哺乳動物とは同種又は異種である。【選択図】なし
