A method for the production of human beta defensin 1 & 2 (HBD-1 and HBD-2) in plant and bacterial cells having antimicrobial activity comprising the steps of: molecular cloning of HBD-1 and HBD-2 in plant expression vector comprising efficient plant promoter, molecular cloning of HBD-1 & HBD-2 in bacterial expression vector to express as fusion protein subjecting the clones of transgenic plant & bacteria to the step of characterization enriching plant derived recombinant proteins purifying the bacteria derived HBD-1 and HBD-2 determining the antibacterial activity of plant and bacteria derived HBD-1 and HBD-2 individually and in combination synergistic antimicrobial activities were observed for both plant/ bacteria derived HBD-1 and HBD-2 when they were used in combination.
