An apparatus and kit for making holes of controlled depth and different diameters on chondral and osteochondral surfaces, comprises: a reamer member (2) having a cutting head (3) for removing tissue from said chondral and osteochondral surface (A), said member (2) being mobile approaching the chondral and osteochondral surface (A) for arranging the head (3) at said tissue; guide means (4) for guiding the reamer member (2) that can be firmly associated with the chondral or osteochondral surface (A), said reamer member (2) being slidable along said guide means (4); and mechanical means (5) for controlling the depth of the hole interposed between the reamer member (2) and the guide means (4) for defining an end stop of the reamer member (2) in the respective movement towards said chondral and osteochondral surface (A).