This invention relates to an apparatus for drug screening or testing comprising: (a) a transparent container containing a subject for placing a stem cell (b) a microdialysis probe having a channel with a perfusate input and a dialysate output, the channel including a dialyzing membrane and located inside the container (c) a continuous source of perfusate connected to the perfusate input and (d) a container for collecting dialysate connected to the dialysate output.This invention also relates to a method for drug screening or testing comprising: (a) placing a stem cell in a transparent container of an apparatus described above (b) contacting a candidate compound with the stem cell under liquid environment by a microdialysis probe (c) collecting dialysate from dialysate output of the microdialysis probe and (d) analyzing the dialysate and/or observing the stem cell to select the candidate having predict effect on the stem cell.本發明係關於一種用於藥物篩選或者測試的裝置,包括:(a)一透明容器,其中包含可放置幹細胞的主體;(b)一微透析探針具有一通道與灌注液入口和透析液出口,且該通道包括一層透析膜並位於該容器內;(c)一灌注液之連續來源,連接該灌注液入口;及(d)一容器,連接該透析液出口用以收集透析液。本發明另關於一種用於藥物篩選或測試的方法,包括:(a)將幹細胞置於上述裝置之透明容器中;(b)使待測化合物與該幹細胞透過微透析探針在液態環境下接觸;(c)自該微透析探針之透析液出口收集透析液;及(d)分析透析液和/或觀察該幹細胞以篩選對該幹細胞有預期效果之待測化合物。(1)‧‧‧導引管(2)‧‧‧透析膜(3)‧‧‧培養基(4)‧‧‧有蓋透明容器的蓋子(5)‧‧‧有蓋透明容器本體(6)‧‧‧PE-10(7)‧‧‧底部流通管(8)‧‧‧通道(9)‧‧‧灌注液入口(10)‧‧‧透析液出口(11)‧‧‧孔洞(12)‧‧‧孔洞(13)‧‧‧自動泵浦(14)‧‧‧自動樣品收集器(15)‧‧‧共軛顯微鏡(16)‧‧‧HPLC-ED系統
