A tissue fastening apparatus related to the present invention includes； a tissue fastening tool formed of a wire wound into a coil shape provided with a first tissue fixing section which is hooked onto a first biological tissue and a second tissue fixing section which is hooked onto a second biological tissue； a tubular puncturing tool which extends a tissue fastening tool and accommodates the stretched tissue fastening tool inside of the puncturing tool； a fastening tool pusher in which the distal end thereof is inserted into the puncturing tool and dispenses the tissue fastening tool inserted into the puncturing tool from a distal end of the puncturing tool when the fastening tool pusher advances； and a rotating mechanism to rotate the puncturing tool when the fastening tool pusher advances.
