Methods for conveying Aphis glycines resistance (RAG) into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing RAG into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with RAG. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance to Aphis glycines; soybean plants, seeds, and tissue cultures produced by any of the disclosed methods; seed produced by the disclosed soybean plants; and compositions including amplification primer pairs capable of initiating DNA polymerization by a DNA polymerase on soybean nucleic acid templates to generate soybean marker amplicons.
