Tan Eugene,Lam Heung Chuan,Bogdanov Valery Leonidovich,Kellogg Gregory John,Wright John A.,Thomann Ulrich Hans,Selden Richard F.
申请号：
US201815894630
公开号：
US2018169660(A1)
申请日:
2018.02.12
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.
