The instant disclosure provides a probe cover dispensing device including a base and an elastic element. The base includes a housing unit, a probe cover dispensing unit and a connection unit disposed between the housing unit and the probe cover dispensing unit. The probe cover dispenser unit can rotate between a first predetermined position and a second predetermined position relative to the housing unit through the connection unit. The elastic element is disposed in the housing unit to apply a pressure on a probe cover which is disposed in the housing unit. The probe cover is abutted by the elastic element and limited in the housing unit. When the probe cover dispensing unit rotates from the first predetermined position to the second predetermined position, a sliding groove may be formed between the housing unit and the probe cover dispenser unit for the probe cover to slide therein.