INHIBITION OF ALDOSE REDUCTASE BY NATURAL COMPOUNDS (AGNUSIDE, EUPALITIN- 3-O-GALACTOSIDE, PICROSIDE II AND 7-O-METHYLWOGONIN) TO TREAT DIABETIC RETINOPATHY
Identification of specific Aldose reductase inhibitors (ARTs) has gained importance in research since most of the tested ARIs were withdrawn due to adverse side effects, mainly due to the nonspecific binding of the ARIs to the Aldoketo reductase (AKR) superfamily proteins, having a high rate of structural similarity to Aldose reductase (ALR2). The phytpcompounds, Agnuside, Picroside II, Eupalitin-3-O-Galactoside and 7-O-Methylwogonin showed potent inhibition of human ALR2 in diabetic lens and ARPE-19 cell line. The Phytocompounds, Agnuside and Eupalitin-3-O-Galactoside inhibited human lens ALR2 with IC50 values lower than the drug Epalrestat (98 nM). Agnuside had the lowest IC50 of 22.4 nM followed by Eupalitin-3-O-Galactoside having an IC50 of 27.3 nM in inhibiting Human ALR2. 7-O-Methylwogonin had an IC50 of 108 nM, and Picroside II had an IC50 of 130 nM in inhibiting ALR2.
