In peritoneal dialysis, a therapy for end stage renal disease patients, glucose has been used mainly as an osmotic agent in the dialysate. However due to the oxidation and decomposition of glucose, protein cross linking reaction progress to cause peritoneum sclerosis, resulting in cease of this therapy The present invention relates to the dialysate comprising protein cross linking suppressor(s) and/or protein cross linkage splitter(s) for the solution of the problem. Effective suppressors or splitters may be reductants, anti-oxidants, mercapto compounds, sulfide, hydrosulfide, salt of reductive sulfur oxy-acid, thiourea and its derivatives, hydroxyl and/or carboxyl residue(s) containing cyclic compounds flavonoids, nitrogen containing heterocyclic compounds, hydrazyl compounds and uronic acid containing mucopoly- saccharides and the like. The dialysate is prepared by sterilizing the dialysate under high temperature and pressure separately from the suppressors and by adding the suppressors, that is sterilized separately, into the dialysate. By use of the dialysate in the present invention, peritoneal dialysis can keep on without suffering from problem.
