PROBLEM TO BE SOLVED: To provide more effective therapeutic means of cancer via PSF1 gene silencing activity.SOLUTION: The invention relates to a composition having PSF1 gene silencing activity and containing a component (I) with a peak at 11.1 minutes in retention time and/or a component (II) with a peak at 14.6 minutes in retention time on the basis of HPLC analyses in the conditions of the following (A)-(F): (A) HPLC column: the column filled with octadecyl silica gel support (particle diameter 3 μm) and having an inside diameter of 4.6 mm and a height of 150 mm (B) HPLC column oven temperature: 40°C (C) flow rate: 0.8 ml/minute (D) detection: 190-400 nm (E) mobile phase: (a) methanol and (b) 10 mM of ammonium formate aqueous solution and (F) gradient condition: maintaining a mixed solvent of 85% of (a) and 15% of (b) for 5 minutes, subsequently linearly increasing the concentration of the (a) in the mixed solvent from 85% to 100% over 15 minutes, and then maintaining the concentration of the (a) 100%.SELECTED DRAWING: NoneCOPYRIGHT: (C)2016,JPO&INPIT【課題】PSF1遺伝子の発現抑制活性を通じた、より効果的ながんの治療手段を提供すること。【解決手段】下記(A)~(F)の条件でのHPLC分析において、保持時間11.1分にピークを有する成分(I)、及び/又は、保持時間14.6分にピークを有する成分(II)を含有し、PSF1遺伝子発現抑制活性を有する組成物:(A)HPLCカラム:オクタデシル化シリカゲル担体(粒子径3μm)を充填した内径4.6mm、長さ150mmのカラム(B)HPLCカラムオーブン温度:40℃(C)流速:0.8ml/分(D)検出:190~400nm(E)移動相:(a)メタノール及び(b)10mMギ酸アンモニウム水溶液(F)グラジエント条件:85%の(a)と15%の(b)との混合溶媒を5分間保持し、その後15分かけて混合溶媒中の(a)の濃度を85%から100%に直線的に上昇させ、その後(a)の濃度を100%に維持。【選択図】なし
