An electron beam irradiation device which can irradiate an electron beam uniformly to an entire outer surface of an object of irradiation by using a small-sized low-energy electron accelerator with a narrow irradiation window, maintain reliability and safety of a sterilization effect high by making a sterilization level of each portion substantially equal, keep the initial cost and the maintenance cost of the device low, and by extending the cost and a usage limit (life) of the electron accelerator is provided. The device has electron beam irradiation means forming an electron beam irradiation zone and gripping/moving means gripping a part of an object of irradiation and causing the object of irradiation to pass through the electron beam irradiation zone, and the whole surface of the object of irradiation can uniformly pass through the electron beam irradiation zone by combining re-gripping of the object of irradiation by two gripping mechanisms provided on the gripping/moving means, rotation of the object of irradiation by two rotation mechanisms, and movement of the object of irradiation by two moving mechanisms.