The present invention relates to an expression kit (1) for extraction of breast milk from a female breast, comprising a funnel (2) for receiving the breast therein, a housing (4), a container (5) for receiving the breast milk, a connection port (7) for connection to a vacuum source, and a membrane element (6) arranged in the housing (4) between the funnel (2) and the container (5) for sealing the funnel (2) with respect to the connection port (7), the funnel (2) with respect to the housing (4) and the housing (4) with respect to the container (5).