CHU NANTES;INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM);UNIVERSITE DE NANTES;ASSOCIATION FRANCAISE CONTRE LES MYOPATHIES
发明人:
BRUMENT, NICOLE
申请号：
CA2975569
公开号：
CA2975569A1
申请日:
2016.02.09
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The invention describes a method for obtaining purified recombinant Adeno-Associated Virus particles (rAAV), comprising the steps of: a) performing a depth filtration of a starting material previously obtained from cells producing rAAV particles, the said starting material being selected in a group comprising a cell lysate and a culture supernatant, whereby a rAAV-containing clarified composition is provided； b) submitting the rAAV-containing clarified composition to an affinity purification step, whereby a first rAAV enriched composition is provided； c) submitting the first rAAV enriched composition at least once to: cl) a step of anion-exchange chromatography on a chromatographic support wherein elution is performed by using a salt gradient, preferably a linear salt gradient, and wherein the rAAV-containing fraction is collected, whereby a second rAAV enriched composition is provided； or c2) a step of density gradient centrifugation, wherein the rAAV-containing fraction is collected, whereby a second rAAV enriched composition is provided； d) submitting the second rAAV enriched composition to a step of tangential flow filtration, whereby purified recombinant Adeno-Associated Virus particles (rAAV) are provided.
