NATIONAL PINGTUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY
发明人:
CHANG, CHI I,张志益,張誌益,HSU, JUE LIANG,徐睿良
申请号：
TW103140584
公开号：
TWI516273B
申请日:
2014.11.24
申请国别(地区):
TW
年份:
2016
代理人:
摘要：
The present invention provides a kind of short chain victory peptide for inhibiting angiotensin converting enzyme, can reach 7.96 μM and 13.30 μM to the IC50 of what angiotensin converting enzyme, can be prevented, treated with what and angiotonin converting Enzyme related symptoms. The present invention separately closes a kind of victory peptide preparation method of what, and (1) provides one buffer of a balsam pear extract liquor what； (2) it provides multiple protein enzyme and reaction is hydrolyzed,； (3) hydrolysis product saves 3000 dongle of molecular weight with membrane filtration and gulps down following product, then with high-performance liquid chromatograph (HPLC) separation. 本發明提供一種抑制血管收縮素轉化酶的短鏈胜肽，其對於血管收縮素轉化酶的IC50可達到7.96μM及13.30μM，可用於預防、治療與血管收縮素轉換酶相關症狀。本發明另關於一種胜肽製備方法，(1)提供一苦瓜萃取液於一緩衝液；(2)提供多種蛋白酶進行水解反應，；(3)水解反應產物以濾膜過濾保存分子量3000道爾吞以下之產物，再以高效液相層析儀(HPLC)分離。
