An apparatus for detecting surface microcirculation of an acupoint includes an acupoint locator, a bio-optical blood flow detection device and a signal analysis processor. The acupoint locator includes an optical probe disposed therein and is configured to let the optical probe substantially align with and be in contact with a surface of the acupoint of a human subject. The bio-optical blood flow detection device is configured to capture microcirculation signals of the surface of the acupoint of the human subject by the optical probe. The signal analysis processor is configured to receive and analyze the microcirculation signals.