Artificial insemination device (1A, IB, IB′;, IB″;, IB′;″;, 1C, ID, IE, IF) for depositing sperm (15) at the cervix entrance of a subject, the device comprises: a longitudinal tube (3) for accommodating sperm (15), and a flexible guiding means (2A, 2B, 2B′;, 2B″;, 2B′;″;, 2C, 2D, 2E, 2F) connected to the tube (3), where the guiding means is arranged for being attached to an insertion unit (7) in order to facilitate at least partly insertion of the tube (3) into the vagina by means of the insertion unit (7).