An aseptic manipulation system comprises first and second operation chambers, an aseptic manipulation chamber, and a control unit. The control unit controls the ventilation of the first operation chamber for more than a first specified number of times, after an object is introduced into the first operation chamber, and ventilate the second operation chamber for more than a second specified number of times that is greater than the first specified number of times, after the object is transferred into the second operation chamber from the first chamber.
