A treatment instrument for endoscope of the present invention includes a puncture needle having a barb. A lymph node removing method of the present invention includes: an insertion step, wherein an endoscope has an insertable insertion portion which is inserted into an esophagus or a trachea being a lumen inside the body of a subject the step of inserting a distal end portion arranged at the tip of the insertion portion of the endoscope into the lumen a confirmation step of confirming the position of a mediastinal lymph node by performing ultrasound scanning using an ultrasound transducer arranged at the distal end portion an anchoring step of placing an anchoring device which has been inserted through a channel inside the insertion portion of the endoscope, on the mediastinal lymph node by performing the ultrasound scanning a incision step of cutting a wall of the lumen to make a fistula and a drawing-in step of drawing the mediastinal lymph node into the lumen with the anchoring device.