A method of identifying iris includes: providing incident beams entering an eye locating at a reference position; setting a first, a second and a third reference point for locating the eye at the reference position; forming a first, a second and a third measuring glint by the incident beams after the eye moves from the reference position to a measuring position, and positions of the first, the second and the third measuring glint corresponding to the positions of the first, the second and the third reference point; capturing an eye image including a first, a second, a third measuring glint image and an iris image; comparing the gray scale value with a threshold gray scale value to obtain the positions of the first, the second and the third measuring glint; and calculating a first and a second variation to obtain an resolution variation of the iris image.