A microscopy system includes a first laser emitting a first laser pulse, the first laser pulse being a pump beam； a second laser emitting a second laser pulse, the second laser pulse being spectrally isolated for generating a probe beam and a donut beam； an optical device for combining the pump beam, the probe beam and the donut beam into a combined laser pulse, the probe beam and donut beam having a phase difference that causes a reduction of a focal volume of the combined laser pulse； a galvanometer scanning system for delivering the combined laser pulse to a focal spot in a focal plane, wherein the reduction of the focal volume of the combined laser pulse initiates a stimulated emission of a targeted molecule, the stimulated emission having dipole-like backscatter； and a sensor for enabling imaging of the dipole-like backscatter.
