A method for the treatment of breast milk, which includes at least two cycles, each cycle includes subjecting the medium to a maximum pressure of between 200 and 400 MPa with a compression speed of between 0.5 and 8 MPa·s−1 and a decompression speed of between 0.5 and 100 MPa·s−1, the initial temperature of the medium being between 25° C. et 50° C. Also, the use of the method, the resulting product and the use thereof in order to meet the nutritional and metabolic requirements of premature babies and nursing infants.