The present invention relates to a guider for separating a tube, and more particularly, to a guider for easily separating a tube and a catheter which are coupled to each other. The guider for separating a tube according to an exemplary embodiment of the present invention may include: a cylindrical body portion which includes a through space which is opened at both ends thereof and formed to enable a tube to penetrate therethrough; and a wing portion which is connected to an outer surface of the body portion and transmits rotational force to the body portion.