GOUDA, Hideki,ISODA, Takeshi,WATANABE, Yasuhiro,GONDA, Kohsuke,OHUCHI, Noriaki,WATANABE, Mika
申请号：
EP15770205
公开号：
EP3124969A4
申请日:
2015.03.23
申请国别(地区):
EP
年份:
2017
代理人:
摘要：
The present invention provides a method capable of more accurately quantifying a biological material expressed on the cell membrane in pathological samples. The present invention is directed to a method for quantifying a biological material (target biological material) expressed on the cell membrane, the method including the steps of: (1a) immunostaining the target biological material with a fluorescent material (1b) immunostaining another biological material (reference biological material) on the cell membrane with another fluorescent material (2) using immunostaining images for the target and reference biological materials to identify the fluorescence signal corresponding to the target biological material and to measure the fluorescence signals corresponding to the target and reference biological materials and (3) correcting the measured value of the fluorescence signal corresponding to the target biological material by a given method to quantify the expression level.
