PROBLEM TO BE SOLVED: To provide an efficient method for growing the seedlings of a plant belonging to the genus Vaccininum by using a micro-propagation technic.SOLUTION: The multibud forms are obtained by implanting the pieces of stem top point tissue of a plant belonging to the genus Vaccininum on a medium added with a plant growth-adjusting substance, and tissue-culturing under a hermetically closed condition. The proliferation and healthy seedling-formation of the obtained multibud forms are performed while controlling the ventilation of a container from the hermetically closed state to an opened up state. From the healthy seed-formed multibud shoots, definite length ears for transplanting are collected, and by transplanting them on cell trays, the rooting is induced under a culturing chamber environment. The rooted seedling trees are acclimatized completely to an outside air environment. Here, basically, in the later stage of proliferation of the multibud forms, the environment for the healthy seedling formation is transferred to the open up environment under an aseptic or a sterilized condition for performing the healthy seedling formation. Thereby, the efficient way up to a rooting induction process and the growth of the healthy seedlings are aimed at so as to make the transfer to the rooting and acclimatization processes easy.COPYRIGHT: (C)2008,JPO&INPIT【課題】マイクロプロパゲーション手法を用いたスノキ属植物の効率的な育苗方法を提供する。【解決手段】スノキ属植物の茎頂点組織片を植物成長調節物質添加培地に着床し、密閉状態で組織培養して、多芽体を得る。得られた多芽体を気密状態から開放状態へと容器の換気を制御しながら、無菌または減菌下で増殖と健苗化を行う。健苗化された多芽体シュートから、一定長の挿し穂を採取、セルトレーに移植して、培養室環境で発根を誘導する。発根した苗木は、外気環境で本格的に順化する。本発明では、基本的に多芽体増殖工程の後段で無菌または減菌下開放環境に移行し、健苗化を行う。これにより、発根誘導工程までの効率化と健全な苗の育成をはかり、発根、順化工程への移行を容易にする。【選択図】図1
