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Nucleic acid structure
专利权人:
独立行政法人科学技術振興機構
发明人:
新海 征治,長崎 健,川津 猛,柿本 真司
申请号:
JP2006529032
公开号:
JP5561893B2
申请日:
2005.07.11
申请国别(地区):
JP
年份:
2014
代理人:
摘要:
To provide a new technique by which efficient transfection is ensured in delivering a target gene into a cell, disclosed is a nucleic acid construct for nuclear import, which comprises a ternary complex consisting of a nucleic acid substance containing a gene to be delivered into the nucleus of a cell, an importin protein (for example, importin-²) capable of passing through the nuclear pore and involved in the nuclear transport, and a binding substance (for example, polyethyleneimine) bound to both of the nucleic acid substance and the importin protein. Nucleic acid transport from outside of a cell into the cell nucleus can be particularly promoted by administering the nucleic acid construct bound to a cell membrane receptor binding factor and/or a membrane fusing substance, or administering the nucleic acid construct encapsulated in a non-viral vector (for example, Sendai virus envelope) having cell membrane permeability and membrane fusing properties.
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中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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