GARDIKIS, Konstantinos,KOUTSIANAS, Nikolaos,PATERA, Anna,DRAGANI, Panagiota,TSOUKALAS, Anagnostis- Ioannis,LETSIOU, Sofia
申请号：
EP20150816858
公开号：
EP3380081(A1)
申请日:
2015.11.23
申请国别(地区):
欧洲专利局
年份:
2018
代理人:
摘要：
Method for preparing a stable controlled-release propolis colloidal dispersion system, wherein propolis -after being frozen for 24 hours- is micronized and then dispersed in the solvent system, which is under stirring and consists of deionized water at 25 °C and either natural 1,3-propanediol or glycerol at a mixture ratio 1,3-propanediol (or glycerol)/water from 15%/85% to 80%/20%, in which deionized water hydroxypropyl-β- cyclodextrin or β-cyclodextrin has been predissolved το 2-10% w/w, and as the propolis/cyclodextrin/solvents system is under intense stirring for 30-45 min at a temperature 20°C to 38°C, a liposomal suspension is added thereto at a ratio of 0.3 to 3.5% w/w, and stirring is performed for 1 to 4 hours under continuous measurement of the total content in polyphenols and of the encapsulation efficiency %, until the specific parameters reach the desired values so that the stirring process is ended and the colloidal system is filtrated through suitable filters, its pH is adjusted and the
