An animal urine/specimen collection device for home or clinic that allows easy, prolonged use and is leak resistant. A disposable urine collection device and marking deterrent for use by pet owners and veterinarians on animals such as dogs is disclosed. The urine collection device of the present invention is formed from one pouch of flexible plastic material formed into a valve opening at the bottom, being inserted into another pouch of plastic flexible material with no valve formed at the bottom, creating an encapsulating container for liquid that passes through the inner valve pouch due to the outer and inner (valve shaped) pouch layers being sealed at the top peripheral edges. Flexible supporters that are integrally formed to the collection device are drawn up to secure on the back near the hind quarters for male animals, and around the legs and thighs, then on the back for female animals.