The present invention relates to the field of dynamic spectroscopy and more precisely to a method involving dynamic molecules spectroscopy technology designed to determine transitional changes in molecules conformation and assemblies both in physiologic and pathologic conditions. The method comprises in vitro fingerprints of a sample taken under highly controlled temperature in order to obtain precise images of either one or an ensemble of molecular dynamics. Due to its precise information, the method according to the invention allows shortening of the drug discovery stage.
