The present disclosure relates to a method for separation of solid particles from a fluid body. Preferably, the present disclosure relates to a method, wherein a unique combination of chemicals including but not limiting to coagulant(s) and flocculant(s) are employed for said separation of solid particles, wherein suitable examples of solid particles are living organisms, including autotrophs such as phototrophs, which are either microscopic or macroscopic in nature. The disclosure thus particularly relates to method of chemical coagulation and flocculation for separating solid particles from a fluid body. The present disclosure also provides for an alternate method, wherein the aforesaid method of coagulation and flocculation is combined with electro-coagulation and/or pH modulation strategies for further optimization to separate said solid particles.