The present invention relates to a honeybee pollen composition, and more particularly to a honeybee pollen composition prepared by mixing solidified honeybee pollen with distilled water and an admixture consisting of a dispersant, a preservative, a thickener, an antioxidant, or a neutralizer and then performing pulverization with a particle size of 100 to 500 nm, resulting in an efficient disruption of cell walls of the honeybee pollen and making the composition into a formulation available for easier ingestion in the body or skin, where the admixture, including a dispersant, a preservative, a thickener, an antioxidant, a neutralizer, etc., is added to the solidified honeybee pollen in the pulverization process to increase the stability, antioxidant effects and polyphenol content of the honeybee pollen composition, thereby acquiring availability of the composition as a cosmetic material or a pharmaceutical composition.